Fungal genomes (ONT)
Service OverviewIn fungal genome studies, genome survey, fine genome and pene-complete genome of fungal can be provided depending on specific research goal. Genome sequencing, assembly and functional annotation can be achieved by combining NGS and TGS or NGS, TGS and Hi-C technologies.
Library construction and sequencing
After-sale technical support
Predicted genes from assembled fungal genome is annotated against KOG database in order to classify gene products into orthologous groups.
Common and unique gene analysis
In comparative genomics analysis, unique gene family of the fungi is identified by protein sequence based classification on OrthoMCL.
Signal peptide prediction
Signal peptide is a short peptide on newly synthesized proteins, which guides protein towards secretory pathways. The existence of signal peptide is predicted based on the protein sequences.
Orthologous genes and protein sequences of a closely related species are identified by blastn mapping between the two genomes. Collinearity at nucleus-level is obtained based on the loci of each orthologous gene on both genomes.
1How to assemble fungi genome data from NGS and TGS?
Answer: There are three assembly strategies. The best assembled genome will be chosen from the three as final outcome of genome assembly. (1) Mixing sequenced fragments from NGS and TGS for assembly. (2) Assembling reads from NGS and TGS to contigs separately and mixing the contigs for further genome assembly. (3) Assembling reads from NGS to contigs and assembling tht contigs into scaffold based on TGS data.
2What is complete genome of fungus?
Answer: Pene-complete genome of fungus is achieved by assembly of NGS, TGS and Hi-C data, where sequences can be assembled into chromosome-level. It doesn't include anchoring scaffolds onto chromosomes based on optical maps. However, the number of assembled scaffolds from this analysis is very close to the number of chromosomes.
3Is there any cautions in sampling for this analysis?
Answer: Firstly, there shouldn't be any contaminated microbials. Nucleus staining is required to identify if the fungi is mononucleus or poly nucleus. Genome survey is recommended to find out the heterozygosity and repetitive ratio to guide sequencing strategy for fine genome.