Bacteria Complete Genomes (ONT)

Bacteria Complete Genomes (ONT)

Service Overview

Biomarker Technologies provides sequencing service on constructing complete genome of bacteria with zero gap. Main workflow of bacteria complete genomes construction includes third generation sequencing, assembly, functional annotation and advanced bioinformatic analysis fulfilling specific research goals. A more comprehensive profiling of bacteria genome empowers revealing of fundamental mechanisms underlying their biological processes, which could also provide valuable reference for genomic researches in higher eukaryotic species, etc.


Project Workflow

Experiemental design
Sample extraction
Library construction and sequencing
Data analysis
After-sale technical support
Sample requirements:

DNA samples: Conc. ≥20 ng/μl (Qubit). Total amount: ≥15 μg (Qubit); Single band on electrophoresis gel; limited degradation.
Culture samples: Culture in log phase; Counting of bacteria after centrifugation: 3e10; Collected in endotoxin-free eppendorf; Fast-frozen in liquid nitrogen; Delivered with dry-ice.

Service specifications:

NGS sequencing depth: ≥100X; TGS sequencing depth: ≥100X;
Bacteria complete genome with 0 gap


Service Advantages

50+ skilled experts in analysis
Diverse successful cases
Highly-experienced technical team
3 distributed computer cluster servers
In-depth data interpretation
Optimized reports delivery
Professional after-sale support
Rapid analysis

Results Demo







Raw data quality control

Quality control will be processed on raw sequencing data generated from third generation sequencing platform to generate high-quality sub-reads, which will be applied in following assembly.







Circular bacteria genome in Circos image

Predicted genome features including repetitive sequences, GC content, etc. will presented on whole genome via Circos image. The components and relative positions on genome can be clearly presented on Circos image.














GO Annotation

Predicted gene sequences will be blast against GO database in order to describe the functions of bacteria genes and proteins in terms of biological processes, cellular components and molecular functions.







Phylogenetic tree

Single copy genes, which provide good resolution for evolutionary relationships, are applied as molecular markers for phylogenetic tree construction. The tree is constructed by phyML software.








Pathway analysis on unique genes

Common and unique genes of a species can be identified. It has been widely accepted that these unique genes are more likely to link to specific environmental conditions or evolutionary processes. functional and pathway annotation of these unique genes will be processed according to various database in order to reveal molecular mechanism of specific biological features.

FAQ

Why is NGS data required in bacteria complete genome construction?
Answer: 100 X NGS data is required in this service. Assembly of NGS data will be used to examine if bacteria samples are contaminated. Moreover, NGS data can be used to correct TGS data in order to generate a more accurate and complete genome.
Will plasmids be assembled in this service?
Answer: Some plasmid DNA will be extracted with genome DNA and processed for sequencing. It may generate some plasmids assembly. However, this can not be guaranteed in case of insert length of the library and uncertain number of plasmids.
What is comparative genomic analysis in bacteria genome sequencing?
Answer: In comparative genomic analysis of bacteria genome, common and unique genes between current bacteria and closely related bacteria species will be identified and collinearity between them will be analyzed. Biomolecular-level mechanisms underlying the physiological differences in between can be studied by annotating unique genes.