BGI sequencing platform

BGI sequencing platform

DNBSEQ-G400

DNBSEQ-T7


Sequencing Principle

DEBSEQTM is remarkable for its low amplification error rate and low repetition rate of output data, which is established on a novel sequencing strategy based on DNBs (DNA nanoballs).
Targeted DNA fragments is denatured to single strand DNA and generate single strand circular DNA (sscirDNA) by adding a splint oligonucleotide with a complementary sequence to 5' and 3' terminal ends.
DNBs are generated by hundreds of rounds of rolling circle amplification (RCA) using sscirDNA as template. In this process, all amplifications are processed with original DNA insert as template, which largely avoid accumulation of errors and GC bias. Therefore, sequencing on base of DNBs is much preferable for SNP and indel detection.
A patterned active binding sites and distance are designed on the surface of flow cell. The binding of DNBs to the spots are firstly driven by electrostatic force and stabled by Van der Waals force. The uniformed size of spots enables one-to-one binding of DNBs on the spots and the larger distance between them empowers a non-interference read-out of each single DNB and reduced index hopping. This optimized patterned array largely saturates the flow cell with DNBs, which greatly increased the efficiency of flow cells.
cPAS technology is employed in sequencing process, where fluorescent signals are released while synthesizing and captured by imaging. The fluorescent signals are converted into digital signals for base-calling.

Platform Specifications

Platform
MGISEQ-2000
DNBSEQ-T7
Features Adaptive Ultra-high Throughput
Applications WGS, WES, trancriptome sequencing and more Whole genome sequencing, transcriptome sequencing, targeted panel projects
Flow cell FCL&FCS FC
Lane
4 Lane & 2 Lane 1 Lane
Operation Mode High Throughput Ultra-high throughput
Max. throughput
/run
1440 Gb 6 Tb
Effective reads
/flow cell
1500-1800 M 5000 M
Average run time approx. 38 hours PE150 within 24 hours
Min. read length SE50 PE100
Max. read length SE400 PE150

Platform Performance

Competitive quality of reads

Raw data Q20>96.16%
Raw data Q30>87.86%
No Index hopping

The application of DNB technology in library construction reduces mis-assignment frequency to 0.0001%-0.0004%
Q-score statistics on WGS data of 1000+ Lanes

Comparison on index hopping frequency

Species
Total Base Properly mapped (%) Depth Cov_ratio_10X (%)
Q20 (%)
Q30 (%)
Soybean 24,020,592,600
95.08 97.75
98.15
92.24
Rice 5,257,019,400
89.73 59.73
97.95
91.86
Apple 25,239,060,400
91.08 92.84
98.08
91.98
C.elegans 10,296,120,400
95.73 99.89
97.94
91.6

Successful Cases

Year Publication Journal
2020 Metagenomic approaches in microbial ecology: an update on whole-genome and marker gene sequencing analyses MICROBIAL GENOMICS
2020 Newly emerged immunogenic neoantigens in established tumors enable hosts to regain immunosurveillance in a T cell-dependent manner International Immunology
2020 Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding Lancet
2020

Development of a CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain Science Advances
2019 Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance GigaScience
2020 Geospatial Hotspots Need Point-of-Care Strategies to Stop Highly Infectious Outbreaks: Ebola and Coronavirus Archives of Pathology & Laboratory Medicine
2020 Harnessing adaptive evolution to achieve superior mannitol production by Lactococcus lactis using its native metabolism Journal of Agricultural and Food Chemistry
2019 Clinical Evaluation of Diagnosis Efficacy of Active Mycobacterium tuberculosis Complex Infection via Metagenomic Next-Generation Sequencing of Direct Clinical Samples Frontiers in Cellular and Infection Microbiology
2020 Effect of fructans, prebiotics and fibres on the human gut microbiome assessed by 16S rRNA-based approaches: a review Beneficial Microbes
2020 Investigation of an imported cholera case in China with whole genome sequencing INFECTION GENETICS AND EVOLUTION
2020 Fulminant central nervous system varicella-zoster virus infection unexpectedly diagnosed by metagenomic next-generation sequencing in an HIV-infected patient: a case report BMC Infectious Diseases
2019   Pathogens in Patients with Granulomatous lobular mastitis INT J INFECT DIS
2020 Sphingomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench International Journal of Systematic and Evolutionary Microbiology
2020 The pregnancy-associated spontaneous coronary artery dissection in a young woman with a novel missense mutation in NOTCH1: a case report BMC Medical Genetics
2019 Metagenomic next-generation sequencing diagnosis of peripheral pulmonary infectious lesions through virtual navigation, radial EBUS, ultrathin bronchoscopy, and ROSE Journal of International Medical Research
2020 Complete mitogenome of the invasive land flatwormPlatydemus manokwari Mitochondrial DNA Part B