Whole Transcriptome SequencingNext-generation Sequencing
Service OverviewWhole transcriptome, as the name indicates, means all RNA molecules under specific conditions, including mRNA and non-coding RNA. Whole transcriptome sequencing, also known as “total RNA sequencing” aims at revealing comprehensive regulatory networks at transcriptome level. Taking advantage of NGS technology, sequences of entire transcriptome products are available for ceRNA analysis and joint RNA analysis, which provides the first step towards functional characterization.
Service AdvantagesDiverse sample types acceptable: tissue, cells, mucosa, blood, etc.
Highly skilled in library construction: Lower amount of RNA required for library construction to save your precious samples
Customized bioinformatic analysis available upon your request
Biomarker Technologies has helped our clients with more than 50 publications (accumulated IF >160), which gained us massive experience in project analysis.
1Which library construction strategy is used in whole transcriptome sequencing?
In order to analyse mRNA, miRNA, circRNA and lncRNA simultaneously, 2 libraries will be constructed, including small RNA library and rRNA-depleted strand-specific library. Recommended data volume for both libraries are 10M reads and 16 G data respectively.
2Is there any requirements on tissue samples for whole transcriptome sequencing?
Since RNA is extremely unstable for degradation, tissue should be preserved in RNAlater buffer immediately after sampling following instructions in order to prevent RNA degradation. The samples in buffer needs to be preserved at -80 degree and delivered on dry-ice. Alternatively, tissue can be fast-frozen in liquid nitrogen, preserved at-80 degree and delivered on dry-ice. More specific sample preservation tips are available upon request.