cDNA synthesis kit

cDNA synthesis kit


BiomarkerScript II 1st Strand cDNA Synthesis Kit

Cat. No. : RK02002

Size: 100 RXN
 




Product Description

BiomarkerScript II 1st Strand cDNA Synthesis Kit is designed for cDNA synthesis containing BiomarkerScript II reverse transcriptase and two types of oligo DT primers.

Features and Benefits

1. Thermal stability and high enzyme activity

BiomarkerScript II reverse transcriptase is a recombinant M-MuLV reverse transcriptase, which has much lower RNase H activity while much higher thermal stability. Compared to wild-type M-MuLV, recombinant M-MuLV reverse transcriptase keeps its activity at upto 48 degree, which enables synthesis of first strand cDNA at relatively higher temperature. This can lead to higher production of more specific cDNA. Moreover, RNA secondary structure can be interupted at higher concentration, which largely improves efficiency of reverse transcription.

2. Longer cDNA synthesized

BiomarkerScript II 1st Strand cDNA Synthesis Kit enables synthesis of cDNA upto 10 kb in length, which is suitable for gene cloning.

Applications

First strand cDNA synthesis
Reverse transcription products for qPCR
Reverse transcription products for gene cloning
cDNA library construction